The Good, the Bad, and the Ugly of ROS: New Insights on Aging and Aging-Related Diseases from Eukaryotic and Prokaryotic Model Organisms

Figure 6

Carbonylation and derivatization of a protein amino acid side chain. A scheme for the formation of glutamic semialdehyde from an arginyl residue is depicted as a consequence of an MCO. For detection, the carbonyl group, in this case, glutamic semialdehyde, is subsequently derivatized by 2,4-dinitrophenolhydrazine. The resulting protein 2,4-dinitrophenolhydrazone can be detected by specific monoclonal or polyclonal antibodies [210]. Reproduced with permission from Nyström T. Role of oxidative carbonylation in protein quality control and senescence. The EMBO Journal. 2005; 24 (7):1311-1317. doi:10.1038/sj.emboj.7600599.