Simvastatin Treatment Upregulates HO-1 in Patients with Abdominal Aortic Aneurysm but Independently of Nrf2

Figure 2

Simvastatin upregulates HMOX1 mRNA and slightly increases HO-1 protein in HAoEC. (a) Simvastatin influence on HAoEC viability after 24–48 h of stimulation (MTT assay). (b) mRNA level of HMOX1, GCLM, and NQO1 in HAoEC after simvastatin treatment (1 and 10 μM) for 6 h and 24 h. (c) Immunofluorescent staining of HO-1 (green) and Bach1 (red) in HAoEC after stimulation with simvastatin (1 and 10 μM) for 6 h. (d) Protein level of HO-1 and Nrf2 after 24 h stimulation with simvastatin (1 and 10 μM) in representative Western blot. Data are presented as mean ± SE; , , and ().

(a)

(b)

(c)

(d)