Curcumin Effectively Rescued Parkinson’s Disease-Like Phenotypes in a Novel Drosophila melanogaster Model with dUCH Knockdown

Figure 6

The curcumin treatment reduced the loss of dopaminergic neurons in adult brains caused by the knockdown of dUCH. (a–c) Images show dopaminergic neurons in adult brains stained with an anti-TH antibody. The indicated white boxes enclose the dopaminergic neuron clusters and are magnified on the right side of each panel (A1–C1, A2–C2, and A3–C3). Scale bars represent 100 μm (A–C) and 25 μm (A1–A3, B1–B3, and C1–C3). (d) The number of dopaminergic neurons in PPM1/2, PPL2ab, and PPM3 clusters from 15-day-old fly brains. The loss of dopaminergic neurons decreased in curcumin-treated flies with the knockdown of dUCH (Tukey’s test, and , ). Error bars represent the standard deviation of data. TH > CS (w/Y; +; TH-GAL4/+) and TH > dUCH.IR (w/Y; +; TH-GAL4/UAS-dUCH.IR).