Propofol Protects Rat Cardiomyocytes from Anthracycline-Induced Apoptosis by Regulating MicroRNA-181a In Vitro and In Vivo

Figure 3

Propofol reduced the upregulation of miR181a induced by ADM and inhibited ADM-induced STAT3 activating. The expression of miR-181a in different treatment on H9c2 (a) or primary cardiomyocytes (b) tested by RT-PCR. The combination treatment upregulation of miR-181a was reduced compared to the ADM-alone group. (c-d) Phospho-STAT3, Bcl-2, Bax, Caspase3, and Caspase9 protein levels were measured in different treatment in cardiomyocytes by Western blotting. (e-f) Semiquantitative data from densitometric analysis of phospho-STAT3, Bcl-2, Bax, Caspase3, and Caspase9 are presented as relative ratio of each protein to β-actin. Data are expressed as mean ± SD (). The statistical analysis was performed with Student’s t-test. NS: not significant; and , compared with the ADM group.

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