Oxidative Stress Induces HSP90 Upregulation on the Surface of Primary Human Endothelial Cells: Role of the Antioxidant 7,8-Dihydroxy-4-methylcoumarin in Preventing HSP90 Exposure to the Immune System

Figure 2

(a) Analysis of soluble HSP90 concentrations (pg/ml) by ELISA in culture supernatants from HUVEC treated with H₂O₂ or left untreated. Results are expressed as mean and standard error of the mean (). ; ; ; . (b) Spearman correlation between the percentages of HSP90-positive cells evaluated by cytofluorimetric assay, and the concentrations of soluble HSP90 (pg/ml) in culture supernatants determined by ELISA in HUVEC treated with H₂O₂ or left untreated after 2 and 4 hours of recovery. Spearman correlation was chosen since a non-Gaussian distribution of the data was observed according to the D’Agostino and Pearson omnibus normality test. The two-phase decay was used to obtain the fit.

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