NRF2 Activation Inhibits Both TGF-β1- and IL-13-Mediated Periostin Expression in Fibroblasts: Benefit of Cinnamaldehyde for Antifibrotic Treatment

Figure 3

Knockdown of NRF2 upregulates the expression of periostin in dermal fibroblasts. (a) NHDF cells were transiently transfected with control siRNA or NRF2 siRNA for 24 h, after which whole cell lysates or total RNA were extracted. The expression levels of NRF2 were normalized to that of GAPDH. NRF2 protein and mRNA expressions are depicted. (b, c) NHDF cells were transiently transfected with control siRNA or 5 nM NRF2 siRNA for 24 h and then stimulated with 5 ng/mL TGF-β1 (b) or IL-13 (c). POSTN mRNA expression is depicted. The values were adjusted by GAPDH expression. The same experiments were performed three times. , .

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