Polydatin Protects Diabetic Heart against Ischemia-Reperfusion Injury via Notch1/Hes1-Mediated Activation of Pten/Akt Signaling
Effect of polydatin and DAPT on myocardial Notch1/Hes1 signaling in MI/R-injured diabetic rats. The diabetic animals received MI/R operation in the presence or absence of polydatin or DAPT treatment. The assessment was performed after 3 hours of reperfusion. (a) Bar diagram showing Notch1 ICD nuclear expression. Top images: representative blots. (b) Bar diagram showing nuclear Notch1 ICD intensity. (c) Representative photomicrographs of Notch1 ICD immunofluorescent staining (bar = 5 μm). Red fluorescence shows Notch1 ICD (row 1). Blue fluorescence shows cardiomyocyte nuclei (row 2). Green fluorescence shows cardiomyocytes (α-sarcomeric-actin, row 3). Arrows show Notch1 ICD nuclear localization; original magnification ×400. (d) Bar diagram showing Hes1 expression. Top images: representative blots. (e) Bar diagram showing Pten expression. Top images: representative blots. (f) Bar diagram showing real-time PCR analysis of Pten mRNA level in the myocardium. (g) Bar diagram showing Akt phosphorylation. Top images: representative blots. The results are expressed as the mean ± SEM. samples per group. / versus the sham group, / versus the MI/R + V group, and versus the MI/R + PD group. MI/R, myocardial ischemia-reperfusion; V, vehicle; PD, polydatin.
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