Double Knockout of Peroxiredoxin 4 (Prdx4) and Superoxide Dismutase 1 (Sod1) in Mice Results in Severe Liver Failure

Figure 4

Oxidative stress induced lipid accumulation in DKO hepatocytes. (a) The average viability of isolated hepatocytes from WT, Sod1^−/−, and DKO mice. Cell counting of either live (white columns) or dead cells (black columns), as measured by the Trypan blue exclusion test. (b) Representative blots of PLIN2, CHOP, SOD1, Prdx4, and β-actin in primary cultured hepatocytes. Proteins extracted from the hepatocytes before and 24 h after culturing were subjected to Western blotting. (c) The morphology of primary cultured hepatocytes of all four genotypes. The DKO hepatocytes containing large amounts of lipid droplets are indicated by yellow arrows. Scale bars: 20 μm. (d) Representative images of DAPI- (blue), PLIN2- (green), and Nile Red- (red) stained primary cultured hepatocytes. Scale bars: 20 μm.

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