Mangiferin and Morin Attenuate Oxidative Stress, Mitochondrial Dysfunction, and Neurocytotoxicity, Induced by Amyloid Beta Oligomers

Figure 2

NADPH oxidase inhibitors are neuroprotective against Aβ oligomers. (a) Neurons were treated with Αβ (5 μM) for 60 min, and ROS generation was monitored with CM-H2DCFDA (30 μM). Apocynin (5 nM), DPI (0.5 μM), and EUK-134 (100 nM) reduced Αβ-induced ROS generation in neurons. (b) The toxicity of Aβ oligomers (5 μM) in cultured cortical neurons as measured 24 h later with the LDH viability assay is prevented by the coapplication of Aβ oligomers with NOX inhibitor DPI. Data represent of the CM-H2DCFDA/calcein and LDH signals in cultures, expressed as a percentage of control untreated levels (100%). compared with untreated cells; , compared with Aβ-treated cells.

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