Characterization of the Antioxidant Effects of γ-Oryzanol: Involvement of the Nrf2 Pathway

Figure 3

Nrf2 nuclear translocation induced by oryzanol. HEK-293 cells were pretreated with ORY for 3, 6, and 24 h and then stressed with 100 μM H₂O₂ for 3 h. Nuclear and cytoplasmic fractions were isolated as described in the Material and Methods. (a) Nuclear expression of Nrf2 was assessed by Western blotting and lamin A/C expression was used as loading control. Data are represented as mean ± SD; , , and versus untreated cells, Dunnett’s multiple comparison test. (b) Cytoplasmic expression of Nrf2 was assessed by Western blotting, and tubulin expression was used as loading control. Data are represented as mean ± SD.

(a)

(b)