Oxidative Medicine and Cellular Longevity / 2018 / Article / Fig 2

Research Article

Pro-BDNF Contributes to Hypoxia/Reoxygenation Injury in Myocardial Microvascular Endothelial Cells: Roles of Receptors p75NTR and Sortilin and Activation of JNK and Caspase 3

Figure 2

Overexpression of pro-BDNF in MMECs and its effect on MMEC apoptosis. (a–e) MMECs were transfected with either pro-BDNF or Ad-GFP. Immunostaining, Western blotting, and quantitative analysis showed that the protein expression of pro-BDNF increased in MMECs after transduction with pro-BDNF. (f–h) Transfected cells were exposed to HG and then subjected to a TUNEL assay (f, g) and enumeration of TUNEL-positive cells (h) to evaluate apoptosis. Pro-BDNF overexpression markedly elevated the numbers of TUNEL-positive cells. The data were analyzed by the -test. The error bars represent SEM. as compared with the control group or Ad-pro-BDNF group.

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