Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia

<div>Expression of VDR. Group I (<svg height="8.55521pt" id="M36" style="vertical-align:-0.2063904pt" version="1.1" viewbox="-0.0498162 -8.34882 27.8368 8.55521" width="27.8368pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M495 86L479 114C446 82 419 66 409 66C401 66 401 72 406 97C420 166 436 231 453 297C489 435 454 448 428 448C406 448 384 439 354 422C305 394 222 327 161 247H159L183 345C200 415 194 448 173 448C143 448 82 410 23 351L38 325C64 349 95 371 105 371C111 371 116 365 109 336L25 -4L31 -12C50 -4 77 3 107 9C119 69 132 122 145 168C197 254 321 381 370 381C387 381 393 374 378 305L329 95C309 17 320 -12 345 -12C372 -12 430 19 495 86Z" id="g113-111"></path></g><g transform="matrix(.013,0,0,-0.013,10.158,0)"><path d="M535 323V373H52V323H535ZM535 138V188H52V138H535Z" id="g117-34"></path></g><g transform="matrix(.013,0,0,-0.013,21.421,0)"><path d="M137 343C167 482 260 545 321 574C357 591 397 603 429 609L423 641C382 634 335 622 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transform="matrix(.013,0,0,-0.013,0,0)"><path d="M495 86L479 114C446 82 419 66 409 66C401 66 401 72 406 97C420 166 436 231 453 297C489 435 454 448 428 448C406 448 384 439 354 422C305 394 222 327 161 247H159L183 345C200 415 194 448 173 448C143 448 82 410 23 351L38 325C64 349 95 371 105 371C111 371 116 365 109 336L25 -4L31 -12C50 -4 77 3 107 9C119 69 132 122 145 168C197 254 321 381 370 381C387 381 393 374 378 305L329 95C309 17 320 -12 345 -12C372 -12 430 19 495 86Z" id="g113-111"></path></g><g transform="matrix(.013,0,0,-0.013,10.158,0)"><path d="M535 323V373H52V323H535ZM535 138V188H52V138H535Z" id="g117-34"></path></g><g transform="matrix(.013,0,0,-0.013,21.421,0)"><path d="M153 550H386L412 615L406 623H120L82 318C104 327 142 338 184 338C294 338 347 275 347 187C347 112 305 39 221 39C160 39 119 71 97 89C88 97 80 96 71 90C59 80 50 67 49 57C48 45 52 36 66 23C80 9 123 -12 169 -12C221 -11 288 15 342 59C403 109 431 165 431 225C431 308 366 395 238 395C212 395 165 379 127 364L153 550Z" id="g113-54"></path></g></svg>) was treated with vitamin D3 and then subjected to I/R (<svg height="11.439pt" id="M39" style="vertical-align:-2.15067pt" version="1.1" viewbox="-0.0498162 -9.28833 57.1008 11.439" width="57.1008pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M687 650H462V622C543 612 549 605 530 547C498 447 422 252 372 126H370C302 298 229 492 204 563C188 607 191 615 262 622V650H17V622C77 616 93 608 122 534C180 389 262 172 329 -11H360C436 196 541 450 568 516C606 605 619 614 687 622V650Z" id="g121-84"></path></g><g transform="matrix(.013,0,0,-0.013,8.526,0)"><path d="M135 536C164 536 186 560 186 587C186 617 164 639 136 639C109 639 85 617 85 587C85 560 109 536 135 536ZM252 0V26C188 32 181 38 181 106V451C138 433 90 420 39 412V388C99 379 102 374 102 312V106C102 38 95 32 32 26V0H252Z" id="g121-103"></path></g><g transform="matrix(.013,0,0,-0.013,11.853,0)"><path d="M298 36L289 62C276 55 253 45 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All experiments were performed in synaptosomal fraction in different brain structures. Results are presented as mean ± SE. <svg height="15.0254pt" id="M40" style="vertical-align:-3.4294pt" version="1.1" viewbox="-0.0498162 -11.596 49.3812 15.0254" width="49.3812pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M426 201C426 274 382 328 332 371C297 401 254 437 222 466C193 492 185 514 185 535S204 587 249 587C285 587 314 569 324 561C332 554 340 559 344 562C351 567 362 579 362 595C362 603 359 609 350 618C337 629 309 640 284 640C221 625 182 595 133 541C89 494 54 434 54 369C54 309 84 257 125 217C154 189 195 147 224 119C275 71 291 43 291 15C290 -27 254 -45 221 -45C182 -45 150 -22 142 -17C136 -14 128 -15 121 -20C113 -27 100 -41 100 -52C100 -62 107 -72 120 -82C139 -97 173 -104 189 -104C241 -88 282 -61 327 -12C372 38 425 116 426 201ZM104 413C104 447 118 478 140 508L143 507C139 470 159 432 183 409C202 389 260 337 294 306C356 252 374 202 374 158C374 116 359 79 336 44L334 46C336 72 326 105 315 123C303 143 271 179 193 250C129 310 104 357 104 411V413Z" id="g190-168"></path></g><g transform="matrix(.013,0,0,-0.013,4.855,0)"><path d="M570 304C570 398 525 448 414 448C385 448 343 445 312 434L329 511L321 518C297 504 262 482 244 460L233 411C195 397 159 381 128 358L135 332C160 347 189 360 224 373L111 -147C97 -210 84 -218 17 -231L13 -257L254 -247L259 -218L233 -216C183 -212 177 -202 189 -142L218 -1C238 -10 266 -12 283 -12C351 3 429 48 483 105C543 168 570 242 570 304ZM482 289C482 161 380 33 304 33C278 33 248 51 233 69L303 396C326 400 352 403 369 403C428 403 482 380 482 289Z" id="g113-113"></path></g><g transform="matrix(.013,0,0,-0.013,16.197,0)"><path d="M512 -3V55L134 254V256L512 456V514L75 281V230L512 -3Z" id="g117-91"></path></g><g transform="matrix(.013,0,0,-0.013,27.46,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z" id="g113-49"></path></g><g transform="matrix(.013,0,0,-0.013,33.7,0)"><path d="M113 -12C146 -12 170 11 170 46C170 78 146 103 114 103S58 78 58 46C58 11 82 -12 113 -12Z" id="g113-47"></path></g><g transform="matrix(.013,0,0,-0.013,36.664,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z" id="g113-49"></path></g><g transform="matrix(.013,0,0,-0.013,42.904,0)"><path d="M153 550H386L412 615L406 623H120L82 318C104 327 142 338 184 338C294 338 347 275 347 187C347 112 305 39 221 39C160 39 119 71 97 89C88 97 80 96 71 90C59 80 50 67 49 57C48 45 52 36 66 23C80 9 123 -12 169 -12C221 -11 288 15 342 59C403 109 431 165 431 225C431 308 366 395 238 395C212 395 165 379 127 364L153 550Z" id="g113-54"></path></g></svg> versus I/R and <svg height="15.0254pt" id="M41" style="vertical-align:-3.4294pt" version="1.1" viewbox="-0.0498162 -11.596 53.7382 15.0254" width="53.7382pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M426 201C426 274 382 328 332 371C297 401 254 437 222 466C193 492 185 514 185 535S204 587 249 587C285 587 314 569 324 561C332 554 340 559 344 562C351 567 362 579 362 595C362 603 359 609 350 618C337 629 309 640 284 640C221 625 182 595 133 541C89 494 54 434 54 369C54 309 84 257 125 217C154 189 195 147 224 119C275 71 291 43 291 15C290 -27 254 -45 221 -45C182 -45 150 -22 142 -17C136 -14 128 -15 121 -20C113 -27 100 -41 100 -52C100 -62 107 -72 120 -82C139 -97 173 -104 189 -104C241 -88 282 -61 327 -12C372 38 425 116 426 201ZM104 413C104 447 118 478 140 508L143 507C139 470 159 432 183 409C202 389 260 337 294 306C356 252 374 202 374 158C374 116 359 79 336 44L334 46C336 72 326 105 315 123C303 143 271 179 193 250C129 310 104 357 104 411V413Z" id="g190-168"></path></g><g transform="matrix(.0091,0,0,-0.0091,4.357,-5.741)"><path d="M426 201C426 274 382 328 332 371C297 401 254 437 222 466C193 492 185 514 185 535S204 587 249 587C285 587 314 569 324 561C332 554 340 559 344 562C351 567 362 579 362 595C362 603 359 609 350 618C337 629 309 640 284 640C221 625 182 595 133 541C89 494 54 434 54 369C54 309 84 257 125 217C154 189 195 147 224 119C275 71 291 43 291 15C290 -27 254 -45 221 -45C182 -45 150 -22 142 -17C136 -14 128 -15 121 -20C113 -27 100 -41 100 -52C100 -62 107 -72 120 -82C139 -97 173 -104 189 -104C241 -88 282 -61 327 -12C372 38 425 116 426 201ZM104 413C104 447 118 478 140 508L143 507C139 470 159 432 183 409C202 389 260 337 294 306C356 252 374 202 374 158C374 116 359 79 336 44L334 46C336 72 326 105 315 123C303 143 271 179 193 250C129 310 104 357 104 411V413Z" id="g190-168"></path></g><g transform="matrix(.013,0,0,-0.013,9.212,0)"><path d="M570 304C570 398 525 448 414 448C385 448 343 445 312 434L329 511L321 518C297 504 262 482 244 460L233 411C195 397 159 381 128 358L135 332C160 347 189 360 224 373L111 -147C97 -210 84 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Oxidative Medicine and Cellular Longevity

fig6

Figure 6

Figure 6: Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia 