Hydrogen Sulfide Alleviates Acute Myocardial Ischemia Injury by Modulating Autophagy and Inflammation Response under Oxidative Stress

Figure 4

Autophagy and apoptosis levels increased in SOD1-knockdown neonatal rat cardiomyocytes under glucose deprivation. (a) Quantitative analysis of superoxide levels in NRCMs. (b) Representative blots and quantitative analysis of LC3II/LC3I and S6K phosphorylation and AMPK phosphorylation. (c) Representative images and quantitative analysis of autophagy flux examined by fluorescent mCherry-GFP-LC3 signal (original magnification, ×400). Yellow puncta represent autophagosomes. Free red puncta represent autolysosomes. (d) Autophagy flux in glucose-deprived NRCMs was inhibited by 10 μM chloroquine. Representative blots and quantitative analysis of LC3II/GAPDH are shown. (e) Representative FACS results at 24 h and quantitative analysis of apoptosis levels from 6 to 48 h are shown. Values represent of three independent experiments. , , and . N: normal condition; GD: glucose deprivation.

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