Icariin Improves the Viability and Function of Cryopreserved Human Nucleus Pulposus-Derived Mesenchymal Stem Cells

Figure 3

The antiapoptosis effect of ICA on postthawed NPMSCs. (a) The phase-contrast photomicrograph of NPMSCs (bar = 100 μm). (b) TUNEL staining of NPMSCs (bar = 100 μm). (c) Representative images of cell apoptosis by flow cytometry analysis after Annexin V/PI dual staining. (d) Summary data showing the apoptosis rate in different groups. The cells at the early stage of apoptosis were stained with Annexin V+/PI−, and the cells at the late stage of apoptosis were stained with Annexin V+/PI+. The data are expressed as mean ± SD from three independent experiments. (, versus control, ; versus DMSO).

(a)

(b)

(c)

(d)