(a)
(b)
Figure 8: Deferred GT treatment dissipated the oxidative stress and ET-1 induction in endothelial cells and prevented neointima formation in the ligated arteries. GT (300 mg/kg/day; s.c.; ) or Con (DMSO; ) treatment was deferred for 3 days after ligation and continued for additional 14 days before the arteries were excised and analyzed. (a) LCA and RCA were processed for H&E staining. The quantitation of intima and media areas was as described in Figure 1. Data are means ± SEM from 6 to 8 serial sections from each artery. (b) Arterial tissue sections were subjected to DHE (red) staining, or ET-1 (green) immunostaining, in addition to CD31 immunostaining (green or red as indicated) and DAPI counterstaining. Arrow heads indicate the DHE+ ECs with pink nuclei. Arrows indicate the green ET-1+ ECs. Quantitation was done as described in Figure 1. The percentages of the positive ECs are means ± SEM from 8 (DHE) or 4 (ET-1) serial sections from each artery. Statistical significance was calculated using two-way ANOVA and post hoc Tukey’s tests, , . Yellow dashed lines delineate the internal or external elastic lamellae. Bars in (a) 100 μm and in (b) 25 μm.