A Novel Mechanism of Mesenchymal Stromal Cell-Mediated Protection against Sepsis: Restricting Inflammasome Activation in Macrophages by Increasing Mitophagy and Decreasing Mitochondrial ROS

Figure 4

BMSCs inhibited NLRP3 inflammasome-mediated caspase-1 activation and IL-1β and IL-18 secretion in bone marrow-derived macrophages (BMDMs). BMDMs were primed with LPS (2 μg/ml) for 4 hours, which was followed by incubation with ATP (5 mM) for 0.5 h. BMSCs, in transwell coculture, were added to BMDMs at the ATP stimulation step. At 18 hours, caspase-1 and IL-1β activation was analyzed in lysates of macrophages by Western blot (a, b), and IL-1β and IL-18 secretion in the supernatants was quantified by ELISA (c). Error bars represent the means ± s.e.m. and . . The data are representative of three or more independent experiments.

(a)

(b)

(c)