A Novel Mechanism of Mesenchymal Stromal Cell-Mediated Protection against Sepsis: Restricting Inflammasome Activation in Macrophages by Increasing Mitophagy and Decreasing Mitochondrial ROS

Figure 7

BMSCs increased Parkin-mediated mitophagy and decreased mtROS generation of BMDMs. (a-b) Western blot analysis for caspase-1 and IL-1β in lysates (a, b) of BMDMs incubated for 1 h with Mito-TEMPO (500 μM), which was followed by LPS and ATP. (c) BMDMs stimulated with 3-MA (10 mM) for 6 h were stained with MitoSOX (5 μM) for 30 min and were then analyzed by flow cytometry. Error bars represent the means ± s.e.m. . . The data are representative of three or more independent experiments.

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