Tanshinone IIA Pretreatment Protects H9c2 Cells against Anoxia/Reoxygenation Injury: Involvement of the Translocation of Bcl-2 to Mitochondria Mediated by 14-3-3η

Figure 4

Inhibition of the translocation of Bcl-2 to the mitochondrial fraction upon 14-3-3η expression in H9c2 cells under anoxia/reoxygenation (A/R) injury. Mitochondrial fractions were isolated, and proteins were extracted from H9c2 cells under A/R injury. Representative Western blot results (a) and quantitative analysis (b) showing that 14-3-3η knockdown reduced Bcl-2 translocation from the cytoplasm to mitochondria. VDAC-1 served as a loading control. Values are presented as the from three individual experiments. Representative confocal immunofluorescence images (c) or quantitative analysis (d) showing Bcl-2 translocation from the cytoplasm to mitochondria under A/R injury. This translocation was abolished by silencing 14-3-3η expression under A/R injury. For quantitative analysis, 10 cells were randomly selected and analyzed. Values are presented as the from three individual experiments. (A) versus control group; (B) versus A/R group.

(a)

(b)

(c)

(d)