Association of Impaired Reactive Aldehyde Metabolism with Delayed Graft Function in Human Kidney Transplantation

Figure 1

Changes in ALDH enzymes found by gene array studies. (a) Experimental scheme for biopsies. 10 paired renal biopsies from kidneys that did not develop delayed graft function and 8 paired renal biopsies that developed delayed graft function were obtained. One biopsy was taken prior to transplant at the end of cold ischemia. The second biopsy was taken after transplant and reperfusion 45 minutes after the cross-clamp was removed. (b) Of the genes identified by Ingenuity pathway analysis, several gene families are identified to be involved in reactive aldehyde metabolism. (c), (d) ALDH2 and ALDH7A1 were statistically significant between kidneys that did not develop delayed graft function compared to kidneys developing delayed graft function (). (e) ALDH4A1 nearly reached statistical significance (^#). (f) ALDH1A1, another ALDH family member, did not change between living donor kidneys compared to kidneys that developed delayed graft function. (-) = donor kidneys without delayed graft function; (+) = donor kidneys with delayed graft function.

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