Association of Impaired Reactive Aldehyde Metabolism with Delayed Graft Function in Human Kidney Transplantation

Table 3

Gene array differences from living compared to deceased donor kidney biopsies.

Pathway	Difference in value	Genes only upregulated in living donor	Genes only upregulated in deceased donor	Common
Serotonin degradation	6.59	ALDH4A1, ALDH2, ALDH7A1, ALDH3A2, ADH6, UGT2B7, UGT2B10, UGT2A3, UGT1A9, AKR1A1, SMOX, DHRS4	None	None
Tryptophan degradation	5.54	ALDH4A1, ALDH2, ALDH7A1, ALDH3A2, AKR1A1, DDC, SMOX	None	None
Histamine degradation	5.34	ALDH4A1, ALDH2, ALDH7A1, ALDH3A2, HNMT, ABP1	None	None
Ethanol degradation II	5.03	ALDH4A1, ALDH2, ALDH7A1, ALDH3A2, ADH6, AKR1A1, ACSS2, DHRS4	None	None
NRF-2-mediated oxidative stress response	4.82	AKR7A2, AKR1A1, FTL, NQO2, ABCC2, MAF, GSTA5, SCARB1, FMO1, GSTA1, GSTA2, GSTA3, MGST1, PRKCQ, ACTB, ACTG1, MGST2, MAP2K3, SQSTM1, AOX1, EIF2AK3, EPHX1	UBB, JUNB, DNAJA1, DNA	FOS, JUN, JUND, DNAJA4, DNAJB11, MAFF
Xenobiotic metabolism signaling	4.63	ALDH4A1, ALDH7A1, ALDH3A2, ALDH8A1, FTL, UGT2B7, NQO2, ABCC2, MAF, GSTA5, CYP3A7, HS6ST2, SMOX, FMO1, GSTA1, GSTA2, GSTA3, MGST1, PRKCQ, UGT2B10, UGT8, PPP2R5A, MGST2, MAP2K3, EIF2AK3, UGT1A9	CITED2, MAP3K8, TNF, HSP90AB1, HSP90AA1	None
Noradrenaline and adrenaline degradation	4.33	ALDH4A1, ALDH2, ALDH7A1, ALDH3A2, ALDH8A1, ADH6, AKR1A1, SMOX, DHRS4	None	None
LPS/IL-1-mediated inhibition of RXR function	4.14	ALDH4A1, ALDH7A1, ALDH3A2, ALDH8A1, GSTA1, GSTA2, GSTA3, GSTA5, APOE, MGST1, SLC27A2, ACOX2, ABCC2, CYP3A7, IL1R2, MGST2, SCARB1, NR5A2, HS6ST2, FMO1, SMOX	ALAS1, HMGCS1, TNF	ACSL3, JUN, NR0B2
Glutathoine-mediated detoxification	4.12	GSTA1, GSTA2, GSTA3, GSTA5, MGST1, MGST2, GGH	None	None
Oxidative ethanol degradation	3.98	ALDH4A1, ALDH2, ALDH7A1, ALDH3A2, ACSS2	None	ACSL3

Whole genome array changes before and after kidney transplantation were compared from each donor kidney that did not develop delayed graft function (living donor) with those that did develop delayed graft function (deceased donor) using Ingenuity pathway analysis. The difference in -values between gene arrays sets were the highest for the 10 signaling pathways listed. Specific genes that were only upregulated in living donor kidneys and deceased donor kidneys are listed. Further, genes that were upregulated in both kidneys that developed delayed graft function versus those that did not are also listed.