Counteraction of Oxidative Stress by Vitamin E Affects Epigenetic Regulation by Increasing Global Methylation and Gene Expression of MLH1 and DNMT1 Dose Dependently in Caco-2 Cells

Table 1

Primer sequences used for MS-HRM analysis. LINE-1-rv contains an additional 5 biotin residue for possible pyrosequencing use. Primer sequences were taken from literature as indicated. Amplicon length and CpG content were determined using BiSearch version 2.53 [65] and Ensembl release 86 database [66].

Primer	Sequence (5-3) for MS-HRM	Reference	Amplicon (bp)	CpGs
MLH1-fw	TTTTTTTAGGAGTGAAGGAGG	[67]	123	13
MLH1-rv	AACRCCACTACRAAACTAAA
DNMT1-fw	GGTATCGTGTTTATTTTTTAGTAA	[68]	115	6
DNMT1-rv	ACGAAACCAACCATACCCAA
LINE-1-fw	TTTTGAGTTAGGTGTGGGATATA	[69]	~143–148	~1–10
LINE-1-rv	AAAATCAAAAAATTCCCTTTC