Counteraction of Oxidative Stress by Vitamin E Affects Epigenetic Regulation by Increasing Global Methylation and Gene Expression of MLH1 and DNMT1 Dose Dependently in Caco-2 Cells

Table 2

Shared 48 h treatments by both glucose conditions per parameter.

	MDA	ROS	Superoxide	Expression		Methylation
				MLH1	DNMT1	MLH1	LINE-1
Control	+	+	+	+	+	+	+
25 μM H2O2				+	+	+	+
50 μM H2O2				+	+	+	+
250 μM H2O2	+	+	+
500 μM H2O2	+	+	+
1 mM NAC	+	+	+
250 μM H2O2 + 1 mM NAC	+	+	+
2 μM VAS2870	+	+	+
250 μM H2O2 + 2 μM VAS2870	+	+	+
10 μM vitamin E	+	+	+	+	+	+	+
50 μM vitamin E	+	+	+	+	+	+	+
25 μM H2O2 + 10 μM vitamin E				+	+	+	+
25 μM H2O2 + 50 μM vitamin E				+	+	+	+
50 μM H2O2 + 10 μM vitamin E				+	+	+	+
50 μM H2O2 + 50 μM vitamin E				+	+	+	+
250 μM H2O2 + 10 μM vitamin E	+	+	+
250 μM H2O2 + 50 μM vitamin E	+	+	+
500 μM H2O2 + 10 μM vitamin E	+	+	+
500 μM H2O2 + 50 μM vitamin E	+