Antioxidant Status in the Soleus Muscle of Sprague-Dawley Rats in Relation to Duodenal-Jejunal Omega Switch and Different Dietary Patterns
Table 1
Antioxidant activity and concentration levels in soleus muscle 8 weeks after DJOS (1st column) and SHAM (2nd column) surgery, subjected to 16 weeks of different dietary patterns, and intergroup comparison between DJOS and SHAM study groups (3rd column) using descriptive statistics and results of two-way analysis of variance. Statistical significance was set at .
DJOS
SHAM
ANOVA
Parameter
HF/HF
HF/CD
CD/HF
CD/CD
HF/HF
HF/CD
CD/HF
CD/CD
Group
Op.
Int.
GR (IU/g)
3.61 ± 0.80
14.32 ± 2.94
7.72 ± 1.18
29.90 ± 8.98
9.96 ± 0.81
9.95 ± 0.93
6.57 ± 1.28
8.05 ± 2.63
<0.001
<0.001
<0.001
CAT (IU/g)
31.95 ± 4.57
34.65 ± 3.15
47.31 ± 3.45
39.28 ± 6.63
56.38 ± 13.99
58.93 ± 3.68
63.91 ± 2.19
29.00 ± 3.33
<0.001
<0.001
<0.001
GPX (IU/g)
2.37 ± 0.64
3.27 ± 0.87
4.44 ± 1.27
1.34 ± 0.14
3.26 ± 0.78
5.14 ± 1.02
8.84 ± 0.42
4.43 ± 0.25
<0.001
<0.001
<0.001
GST (IU/g)
1.12 ± 0.08
1.42 ± 0.26
1.47 ± 0.34
0.78 ± 0.09
1.57 ± 0.34
1.38 ± 0.32
1.49 ± 0.34
1.30 ± 0.11
<0.01
<0.01
<0.05
Total SOD (NU/mg)
85.59 ± 2.90
80.72 ± 6.0
105.64 ± 17.55
74.33 ± 1.69
93.16 ± 7.01
93.87 ± 3.24
91.53 ± 4.40
75.55 ± 1.27
<0.001
0.359
<0.001
MnSOD (NU/mg)
24.50 ± 4.05
36.00 ± 3.02
37.67 ± 11.22
27.59 ± 3.36
30.25 ± 2.93
40.93 ± 8.56
38.72 ± 8.33
39.44 ± 5.17
<0.001
<0.01
0.246
CuZnSOD (NU/mg)
55.49 ± 3.42
48.73 ± 1.63
43.30 ± 2.04
35.00 ± 2.92
72.19 ± 8.47
39.97 ± 4.56
53.93 ± 5.64
42.44 ± 5.61
<0.001
<0.001
<0.001
MDA (μmol/g)
4.56 ± 0.24
5.49 ± 1.34
5.57 ± 0.24
3.41 ± 0.30
4.76 ± 0.57
6.82 ± 0.67
5.92 ± 0.36
4.45 ± 0.30
<0.001
<0.001
0.083
GR: glutathione reductase; CAT: catalase; GPX: glutathione peroxidase; SOD: total superoxide dismutase; GST: glutathione-S-transferase; MnSOD: Mn superoxide dismutase; ZnSOD: Zn superoxide dismutase; MDA: malondialdehyde; DJOS: duodenal-jejunal omega switch surgery; HF: high-fat diet; CD: control diet; HF/HF, CD/HF, HF/CD, CD/CD: type of diet 8 weeks before/8 weeks after surgery; Op.: operation type; Int.: interaction between group and operation type. deviation or median (lower – upper quartile).
