Hydrogen Sulfide Abrogates Hemoglobin-Lipid Interaction in Atherosclerotic Lesion

Figure 8

Modulators of CSE expression in HASMCs, macrophages, and endothelial cells. (a) Confluent HASMCs, RAW264.7 cells, and HAoECs were exposed to LDL (50 μg/mL), oxLDL (50 μg/mL), PL (500 μg/mL), oxPL (500 μg/mL), and H₂O₂ (100 μmol/L) for 8 hours. (b) Confluent HASMCs, RAW264.7 cells, and HAoECs were exposed to Hb, oxHb, heme (50 μmol/L heme group each), and Fe-ammonium citrate (50 μmol/L) for 8 hours. (c) Confluent HASMCs, RAW264.7 cells, and HAoECs were exposed to IL-1β (1–5-10 ng/mL) or TNF-α (1–5-10 ng/mL) for 8 hours. Representative Western blots showing CSE expressions of cell lysates (20 μg/lane) (). Immunoblots were reprobed with GAPDH.

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