ATM Induces Cell Death with Autophagy in Response to H₂O₂ Specifically in Caenorhabditis elegans Nondividing Cells

Figure 2

atm-1(tm5027) larvae exhibited sensitivity, but adult worms were resistant to H₂O₂. (a, b) Synchronized L1 larvae of N2 and atm-1(tm5027) underwent time-course treatment with (a) 40 mM MV or (b) 0.5 mM H₂O₂ at 20°C. After treatments, the worms were cultured for 4 days and we calculated the ratio of adult worms/transferred L1 worms. (c, d) Synchronized adult worms of N2 (white bars) and atm-1(tm5027) (gray bars) were treated with (c) 200 mM MV or (d) 88 mM H₂O₂ for 1 hour at 20°C. 24 hours after these treatments, the percent survival was calculated. All data are mean ± SD and means significantly different by Student’s -test (). The photograph of a worm was obtained from TogoTV (© 2016 DBCLS TogoTV).