Effects of Long-Term Treatment with a Blend of Highly Purified Olive Secoiridoids on Cognition and Brain ATP Levels in Aged NMRI Mice

Table 2

Oligonucleotide primer sequences, product sizes, and primer concentrations for quantitative real-time PCR. bp: base pairs; conc: concentration.

Primer	Sequence	Manufacturer	Product size; (bp)	Conc.; (μM)
AMPK (beta subunit)	5-agtatcacggtggttgctgt-3; 5-caaatactgtgcctgcctct-3	Biomol; Hamburg, Germany	190	0.1
B2M	5-ggcctgtatgctatccagaa-3; 5-gaaagaccagtccttgctga-3	Biomol, Hamburg, Germany	198	0.4
CI	5acctgtaaggaccgagaga-3; 5-gcaccacaaacacatcaaaa-3	Biomol, Hamburg, Germany	227	0.1
CIV	5-ctgttccattcgctgctatt-3; 5-gcgaacagcactagcaaaat-3	Biomol, Hamburg, Germany	217	0.1
CS	5-aacaagccagacattgatgc-3; 5-atgaggtcctgctttgtcct-3	Biomol, Hamburg, Germany	184	0.1
GAP43	5 agggagatggctctgctact-3; 5 gaggacggggagttatcagt-3	Biomol; Hamburg, Germany	190	0.15
GPx-1	5-gtccagcgtgtatgccttct-3; 5-ctcctggtgtccgaactgat-3	Biomol, Hamburg, Germany	217	0.1
PGK1	5-gcagattgtttggaatggtc-3; 5-tgctcacatggctgacttta-3	Biomol, Hamburg, Germany	185	0.4
Sirt1	5-gtgagaaaatgctggcctaa-3; 5-ctgccacaggaactagagga-3	Biomol, Hamburg, Germany	161	1
SOD2	5-acagcgcatactctgtgtga-3; 5-gggggaacaactcaactttt-3	Biomol, Hamburg, Germany	183	0.1
Synaptophysin 1	5-tttgtggttgttgagttcct-3; 5-gcatttcctccccaaagtat-3	Biomol, Hamburg, Germany	204	0.1