Oxidative Stress-Mediated Overexpression of Uracil DNA Glycosylase in Leishmania donovani Confers Tolerance against Antileishmanial Drugs

Figure 2

Effect of cofactors on glycosylase activity of recombinant LdUNG and native lysate assayed in the presence of 1, 10, 50, and 100 mM of CaCl₂ (a), MnCl₂ (b), and MgCl₂ (c). Data was analysed using mix model ANOVA, followed by Bonferroni posttests using GraphPad Prism 5.0. Mixed model ANOVA determines how glycosylase activity of LdUNG enzyme is affected by factors (type of LdUNG (recombinant or native lysate) and different cofactors CaCl₂/MgCl₂/MnCl₂). The values tell us that the difference between groups is likely due to the chance or due to the variable in the study. means that there was 0.1% chance that the effect may be due to random variation; ns, nonsignificant. The y-axis in all three graphs corresponds to relative glycosylase activity (mean ± SD) of recombinant LdUNG and leishmania lysate.

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