17β-Estradiol Regulates Microglia Activation and Polarization in the Hippocampus Following Global Cerebral Ischemia

Figure 6

Suppression of M1 phenotype markers by E2 in the LPS-activated BV2 microglia cells in vitro. (a–c) mRNA was collected from control, LPS-activated, and LPS activated + E2-treated BV2 cells at 16 hours after activation and treatment. Quantitative RT-PCR analysis of M1 markers, CD86, iNOS, and CD32, indicates a significant upregulation after LPS activation. This upregulation is significantly suppressed by E2 treatment in the activated cells. (d, e) Western blot analysis and quantification of the M1 marker, iNOS, indicate a significant increase in expression after LPS activation and suppression by E2 treatment (n = 5-6 per group) (, control versus LPS, , LPS versus LPS + E2).

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