Aucubin Protects against Myocardial Infarction-Induced Cardiac Remodeling via nNOS/NO-Regulated Oxidative Stress

Figure 3

Aucubin inhibits inflammation and apoptosis. (a) Immunohistochemistry staining of CD45- and CD68-positive cells in the heart in the indicated mouse hearts post-MI (). (b) The relative mRNA levels of TNFα, MCP-1, and IL-1 in the indicated mouse hearts post-MI (). versus vehicle-sham and versus vehicle-MI. (c) TUNEL staining (left) and quantitation (right) in the indicated mouse hearts post-MI (, versus vehicle-MI). (d) Representative Western blots and quantitation of Bax, Bcl-2, and c-caspase 3 in the indicated mouse hearts post-MI (, versus vehicle-sham and versus vehicle-MI). Left, representative blots; right, statistical analysis result. versus vehicle-sham and versus vehicle-MI H9c2 cells were pretreated with aucubin (1 μM, 10 μM, and 50 μM) for 12 h and then exposure to hypoxia for 24 h. (e) Cell counting kit-8 (CCK8) assays were performed to detect cell viability (, versus hypoxia and versus hypoxia + 1 μM aucubin). (f) TUNEL staining (left) and quantitation (right) in the indicated group (). (c) and (g) Western blots of Bax, Bcl-2, and c-caspase 3 in the indicated group (left, representative blots; right, statistical analysis result) ( sample). versus normoxia + PBS and versus hypoxia + PBS. All experiments were repeated independently three times.

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