Research Article

Possible Involvement of Mitochondrial Dysfunction and Oxidative Stress in a Cellular Model of NAFLD Progression Induced by Benzo[a]pyrene/Ethanol CoExposure

Figure 4

Effects of the antioxidant Tempol on ROS production and mitochondrial respiration in steatotic HepaRG cells exposed or not to ethanol and B[a]P. Steatotic HepaRG cells were untreated (c) or treated with 25 μM Tempol (T), 2.5 μM B[a]P, and 25 mM ethanol (BE) or a combination of the three compounds (TBE) for 14 days. (a) ROS production assessed with the H2DCFDA and MitoSOX Red dyes. (b) Parameters of mitochondrial respiration provided by the XF Cell Seahorse Mito Stress Test profile: basal respiration, maximal respiration, ATP production, and proton leak. Results are for 5 (a) and 3 (b) independent cultures. Significantly different from untreated HepaRG cells. #Significantly different from HepaRG cells treated with B[a]P and ethanol.
(a) ROS production
(b) Mitochondrial respiration