Cytoprotective Roles of a Novel Compound, MHY-1684, against Hyperglycemia-Induced Oxidative Stress and Mitochondrial Dysfunction in Human Cardiac Progenitor Cells

Figure 2

Effect of MHY-1684 on hyperglycemia-induced cell death of hCPCs. (a) hCPCs were treated with 25 mM D-(+) glucose for 0–72 h, and cell viability was subsequently assessed. (b) Following the cotreatment of hCPCs with MHY-1684 (0.1, 1, and 10 μM) and 25 mM D-glucose for 72 h, cell viability was assessed. Values are the mean ± S.E.M. as compared with the control group; as compared with the 25 mM D-(+) glucose-treated group. (c) After treatment with MHY-1684 for 24 h in 25 mM D-(+) glucose, the prosurvival-related proteins (ERK-1, AKT-1) were analyzed by Western blotting. (d) hCPCs were incubated with 25 mM D-(+) glucose for 0–72 h with or without 1 μM MHY-1684 for 72 h. hCPCs were stained with annexin V/PI, and then, hCPCs were measured by flow cytometry. hCPCs were classified as viable cells (annexin V-/PI-), apoptotic cells (annexin V+/PI-, annexin V+, PI+), and dead cells (annexin V+/PI+). (e) Dead cell population is expressed as a percent of the total cell population. (f) Apoptotic cell population is expressed as a percent of the total cell population. Values are the mean ± S.E.M. and as compared with the control group; as compared with the group treated with 25 mM D-(+) glucose for 72 h.

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