Taurine Attenuates Calpain-2 Induction and a Series of Cell Damage via Suppression of NOX-Derived ROS in ARPE-19 Cells

Figure 1

Earle’s balanced salt solution (EBSS) increases NADPH oxidative activity and ROS generation in ARPE-19 cells in a time-dependent manner. (a–c) A Western blot analysis was carried out to detect the expression levels of NOXs. (d, e) Intracellular ROS was determined by flow cytometry. (f, g) Earle’s balanced salt solution (EBSS) increases cytoplasmic calcium in ARPE-19 cells. The intracellular calcium was detected with Fluo-3 AM. TM stands for tunicamycin. The data are presented as the means ± SEM of three independent experiments. and compared to control (0 h).

(a)

(b)

(c)

(d)

(e)

(f)

(g)