Degradation of TRPML1 in Neurons Reduces Neuron Survival in Transient Global Cerebral Ischemia

Figure 3

Autophagy enhancement and apoptosis inhibition by TRPML1 activation. (a–d) Representative immunoblots of beclin-1, LC3, and p62 from cultured hippocampal neurons preincubated with 10 μM ML-SA1 (a, ) or transfected with TRPML1 lentivirus (c, ) 1 hr after OGD. (b) or (d) was the statistics for (a) or (c). (e–h) Representative immunoblots of cleaved caspase3 from cultured hippocampal neurons preincubated with 10 μM ML-SA1 (e, ) or transfected with TRPML1 lentivirus (g, ) 1 hr after OGD. (f) or (h) was the statistics for (e) or (g). β-Actin serves as a loading control. All data were presented as mean ± SEM. All comparisons between groups for statistical significance were performed with one-way analysis of variance (ANOVA) with Tukey’s post hoc test. ,, versus Ctrl. , versus OGD.

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