Palmitoylethanolamide Dampens Reactive Astrogliosis and Improves Neuronal Trophic Support in a Triple Transgenic Model of Alzheimer’s Disease: In Vitro and In Vivo Evidence

Figure 5

Effect of chronic um-PEA on neuronal support and survival in FC of 3×Tg-AD and non-Tg mice. (a) Representative immunoreactive species and Western blot densitometric analysis of (b) BDNF and (c) MAP2. β-Actin was used as loading control. Results are expressed as percentage of the mean control value (non-Tg/placebo). (d) Representative fluorescent photomicrographs of MAP2 (red) staining in FC of 6-month-old non-Tg and 3×Tg-AD mice, placebo- or um-PEA-treated. Nuclei were stained with DAPI (blue). Scale bar is 50 μm. (e) Fluorescence analysis is expressed as ΔF/F₀. Experiments were performed three times in triplicate. Data are presented as mean ± SEM. The statistical analysis was performed by two-way ANOVA followed by Bonferroni’s post hoc multiple comparison test ( versus non-Tg/placebo group; versus 3×Tg-AD/placebo group).

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