Palmitoylethanolamide Dampens Reactive Astrogliosis and Improves Neuronal Trophic Support in a Triple Transgenic Model of Alzheimer’s Disease: In Vitro and In Vivo Evidence

Table 3

Experimental conditions for Western blot from 3×Tg-AD mice astrocytes and FC.

Primary antibody	Brand primary antibody	Dilution	Secondary antibody	Brand secondary antibody
GFAP	Abcam	1 : 50000 5% milk in TBS-T 0.1%	HRP conjugated goat anti-rabbit IgG 1 : 30000 5% milk in TBS-T 0.1%	Jackson ImmunoResearch
S100B	Novus Biologicals	1 : 1000 5% BSA in TBS-T 0.1%	HRP conjugated goat anti-rabbit IgG 1 : 10000 5% BSA in TBS-T 0.1%	Jackson ImmunoResearch
COX-2	Cell Signaling	1 : 1000 5% milk in TBS-T 0.1%	HRP conjugated goat anti-rabbit IgG 1: 10000 5% milk in TBS-T 0.1%	Jackson ImmunoResearch
iNOS	Sigma-Aldrich	1 : 8000 1% BSA in TBS-T 0.1%	HRP conjugated goat anti-rabbit IgG 1 : 10000 1% BSA in TBS-T 0.1%	Jackson ImmunoResearch
BDNF	Santa Cruz	1 : 500 5% milk in TBS-T 0.1%	HRP conjugated goat anti-rabbit IgG 1 : 10000 5% milk in TBS-T 0.1%	Jackson ImmunoResearch
MAP2	Novus Biologicals	1 : 250 5% BSA in TBS-T 0.1%	HRP conjugated goat anti-rabbit IgG 1 : 10000 5% BSA in TBS-T 0.1%	Jackson ImmunoResearch
Aβ(1–42)	Millipore	1 : 1000 5% BSA in TBS-T 0.1%	HRP conjugated goat anti-mouse IgG 1 : 10000 5% BSA in TBS-T 0.1%	Jackson ImmunoResearch
β-actin	Santa Cruz	1 : 1500 5% milk in TBS-T 0.1%	HRP conjugated goat anti-rabbit IgG 1 : 10000 5% milk in TBS-T 0.1%	Jackson ImmunoResearch