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Oxidative Medicine and Cellular Longevity
Volume 2018, Article ID 4932905, 17 pages
https://doi.org/10.1155/2018/4932905
Research Article

Transcriptome Remodeling of Differentiated Cells during Chronological Ageing of Yeast Colonies: New Insights into Metabolic Differentiation

1Faculty of Science, Charles University, BIOCEV, 252 50 Vestec, Czech Republic
2Institute of Microbiology of the Czech Academy of Sciences, BIOCEV, 252 50 Vestec, Czech Republic
3Department of Medical Genetics, Oslo University Hospital and University of Oslo, 0450 Oslo, Norway

Correspondence should be addressed to Zdena Palková; zc.inuc.rutan@panedz

Received 13 September 2017; Revised 8 November 2017; Accepted 13 November 2017; Published 11 January 2018

Academic Editor: Karin Thevissen

Copyright © 2018 Derek Wilkinson et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We present the spatiotemporal metabolic differentiation of yeast cell subpopulations from upper, lower, and margin regions of colonies of different ages, based on comprehensive transcriptomic analysis. Furthermore, the analysis was extended to include smaller cell subpopulations identified previously by microscopy within fully differentiated U and L cells of aged colonies. New data from RNA-seq provides both spatial and temporal information on cell metabolic reprogramming during colony ageing and shows that cells at marginal positions are similar to upper cells, but both these cell types are metabolically distinct from cells localized to lower colony regions. As colonies age, dramatic metabolic reprogramming occurs in cells of upper regions, while changes in margin and lower cells are less prominent. Interestingly, whereas clear expression differences were identified between two L cell subpopulations, U cells (which adopt metabolic profiles, similar to those of tumor cells) form a more homogeneous cell population. The data identified crucial metabolic reprogramming events that arise de novo during colony ageing and are linked to U and L cell colony differentiation and support a role for mitochondria in this differentiation process.