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Figure 1: Subpopulations in 15-day-old colonies. (a) Schematic of subpopulations in 15-day-old colonies. (b) Small subpopulations separated from the central part of the colony. (left) Vertical cross section of a 15-day-old colony. (right) Cell subpopulations separated from the colony for RNA sequencing. Cells were visualized by Nomarski contrast. U: upper; L: lower; U1: upper U cells; U2: lower U cells; L1: upper L cells; L2: lower L cells.