Figure 5: Time point comparisons. UpSet plot of datasets of DE genes in upper (U6 and U15), lower (L6 and L15), and marginal (M6 and M15) cells from 6- and 15-day-old colonies, respectively (upper part). Only major intersections are shown. Heat map of genes assigned to functional categories and clustered according to FC and DE in different sample comparisons (lower part). The higher the number of up-/downregulated genes, the more intense the color. Number in heat map cell = number of genes from FC, up- or downregulated in sample comparison. Red cell highlighting: FC upregulated at day 15 relative to day 6. Blue cell highlighting: FC upregulated at day 6 relative to day 15. Arrows indicate upregulation/downregulation in the respective subpopulation ratio(s).