Research Article

Evodiamine Attenuates P2X7-Mediated Inflammatory Injury of Human Umbilical Vein Endothelial Cells Exposed to High Free Fatty Acids

Figure 3

Effects of EVO on ROS production in HUVECs. (a) High FFAs (1 and 1.5 mM) increased ROS production. (b) HUVECs were cultured with control (1% BSA) or high FFAs (1 mM) in the absence or presence of 0.25 μM EVO for 72 h. ROS production was examined after cells were loaded with 10 μM DCFH-DA (a ROS fluorescence indicator). (c) Extracellular ATP in cell supernatant was significantly increased in HUVECs after the treatment of high FFAs. EVO could decrease ATP release in high FFA-treated cells. Data are mean ± SEM of three independent experiments in triplicate. and versus control group and and versus high FFA group.
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