Rutin Isolated from Chrozophora tinctoria Enhances Bone Cell Proliferation and Ossification Markers

Figure 3

Effect of rutin on mRNA expression of some ossification related genes in SAOS-2 cell line. Cells were incubated with rutin (1 μM) or E₂ (0.1 μM) for 48 h. Total RNA was extracted and subjected to RT-PCR. Data were normalized to β-actin; fold changes were calculated and expressed as mean ± SD; . Significantly different from the control untreated cells; .

(a) Osteocyte markers

(b) Osteoblast markers

(c) Osteoclast markers

(d) RUNX suppressors