Combination of Epigallocatechin Gallate and Sulforaphane Counteracts In Vitro Oxidative Stress and Delays Stemness Loss of Amniotic Fluid Stem Cells

Figure 2

Effect of AFSC treatment with SF and EGCG on the ROS intracellular level. (a) Cells were treated or cotreated for 3 days with 1 μM SF and 10 μM EGCG. ROS levels were evaluated by DCFH-DA fluorometric assay, as reported in Materials and Methods. Each bar represents means ± SEM of 3 independent experiments. Data were analysed by one-way ANOVA followed by Bonferroni’s test. , with respect to relative control. (b) Cells were cotreated for 3 days after their thawing or (c) chronically treated for 25 days. The ROS level was evaluated by DCFH-DA fluorometric assay as reported in Materials and Methods. Each bar represents means ± SEM of 3 independent experiments. Data were analysed by one-way ANOVA followed by Bonferroni’s test. , with respect to relative control.

(a)

(b)