Relevance of Alternative Routes of Kynurenic Acid Production in the Brain

Figure 4

Effect of KAT inhibitor and a ROS scavenger on KYNA production from L-Kyn or D-Kyn in rat brain homogenates. AOAA and NDGA were used at 1 mM and 5 μM, respectively. Homogenates were incubated with (a) L-Kyn or (b) D-Kyn (20 μM and 10 μM of ONOO⁻ in Krebs buffer) during 1 h at 37°C. Data are expressed as a percentage of endogenous tissue levels of KYNA and represent the mean ± SEM of 5 experiments per group. In both cases versus control, versus L-Kyn, and versus L- or D-Kyn + ONOO⁻ (one-way ANOVA followed by Tukey’s post hoc test). AOAA: aminooxyacetic acid; ONOO⁻: peroxynitrite; NDGA: nordihydroguaiaretic acid.

(a)

(b)