Methionine Sulfoxide Reductase B1 Regulates Hepatocellular Carcinoma Cell Proliferation and Invasion via the Mitogen-Activated Protein Kinase Pathway and Epithelial-Mesenchymal Transition

Figure 3

MsrB1 inhibits HCC cell proliferation in vitro and in vivo. (a) The MTT assay showed that MsrB1 knockdown inhibits the proliferation of LM3 and snu387 cells in 24, 48, and 72 h (). (b) The MTT assay showed that MsrB1 knockdown inhibits the proliferation of LM3 and snu387 cells in 24, 48, and 72 h under stress of sorafenib (). (c) Flow cytometry showed that MsrB1 knockdown inhibits the proliferation rate of HCC cells. (d) The EdU assay showed that the proliferation percentage of red-fluorescent cells was lower than that of control cells. (e) MsrB1 knockdown inhibits the colony formation of HCC cells. (f) MsrB1 knockdown inhibits the tumor volumes of the subcutaneous tumor of the two groups in 7 weeks in LM3 cells. (g) The expression of MsrB1 in the tumors of both groups of mice was shown to be much lower in the sh-MsrB1 group than in the sh-NC group.

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