MsrB1 affects proliferation/migration of HCC cells by inhibition of the MAPK pathway, inducing apoptosis and inhibition of EMT. (a) The mRNA expression of MsrA/B2/B3, PCNA, FOXK1, β-catenin, TGF-β1, and MMP2/9 was regulated in MsrB1-knockdown cells. (b) The expression of protein phosphorylation of the key proteins of the MAPK pathway, namely, ERK, MEK, and level of p53, was inhibited, but activation and cleaved PARP and caspase 3 expression levels change reflective proliferation inhibition. (c) MsrB1 knockdown increased E-cadherin expression levels. However, MsrB1 knockdown decreased c-myc, Snail, TGF-β1, Slug, β-catenin, MMP-2, and MMP-9 expression levels, indicating that MsrB1 knockdown inhibited EMT.