Modulation of Oxidative Status by Normoxia and Hypoxia on Cultures of Human Dermal Fibroblasts: How Does It Affect Cell Aging?

<div>Markers of cellular aging. (a) Quantitative analysis of positive <i>β</i>-galactosidase-stained cells in HDF during cellular aging under 5% and 21% O<sub>2</sub> culture conditions. The percentage of blue cells observed in old cells (right panel) under a standard light microscope was calculated. Error bars represent ± SEM. (b) p16 mRNA modulation during aging in old (O) HDF with respect to young (Y) ones cultured under normoxic (21% O<sub>2</sub>) and hypoxic (5% O<sub>2</sub>) conditions; (c) p16 mRNA expression in cells cultured under 21% O<sub>2</sub> with respect to 5% O<sub>2</sub> (right panel). The gene was normalized vs GAPDH/<i>β</i>-actin/SDHA, and the results are reported as normalized fold expression. 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Oxidative Medicine and Cellular Longevity

fig2

Figure 2

Figure 2: Modulation of Oxidative Status by Normoxia and Hypoxia on Cultures of Human Dermal Fibroblasts: How Does It Affect Cell Aging? 