The Role of Nrf2 Signaling in PPARβ/δ-Mediated Vascular Protection against Hyperglycemia-Induced Oxidative Stress

Figure 1

Effects of PPARβ/δ agonists on Nrf2 expression. (a, c) mRNA and (b, d) protein expression of Nrf2 in HUVECs exposed to low (5 mM, LG) or high glucose (30 mM, HG) for 24 h with or without GW0742 (GW) or L165041 (L) alone or preincubated with the PPARβ/δ antagonist GSK0660 (GSK). mRNA data presented as a ratio of arbitrary units of mRNA (2^−ΔΔCt). All data are mean ± SEM (), and experiments were repeated at least three times independently. Protein data presented as densitometric values and protein band normalized to the corresponding α-actin; the bands are representative of –5. versus LG. versus HG. versus L and GW column, respectively.

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