The Role of Nrf2 Signaling in PPARβ/δ-Mediated Vascular Protection against Hyperglycemia-Induced Oxidative Stress

Figure 5

Effect of PPARβ/δ agonists in intracellular ROS production. (a) ROS and (b–d) mRNA expression levels of NOX-4 (b), NOX-1 (c), and NOX-2 (d) in HUVEC transfected with PPARβ/δ-specific siRNA (siRNA PPARβ/δ) incubated in low- (LG) or high-glucose (HG) medium for 24 h in the presence or absence of either L165041 (L, 10⁻⁶ M) or GW0742 (GW, 10⁻⁶ M), respectively. GSK0660 (10⁻⁶ M) was added 30 min before the incubation with L165041. mRNA data presented as a ratio of arbitrary units of mRNA (2^−ΔΔCt). All data are mean ± SEM (), and experiments were repeated at least three times independently. versus LG. and versus HG.

(a)

(b)

(c)

(d)