Transplantation of Bone Marrow Mesenchymal Stem Cells Prevents Radiation-Induced Artery Injury by Suppressing Oxidative Stress and Inflammation

Figure 1

Morphology and features of human bone marrow mesenchymal stem cells (hBMSCs). (a) The morphological features of cultured hBMSCs at the 5th day; the 3rd and 6th passages (P3 and P6) were evaluated by the light microscope or HE staining. (b) Cell cycle analysis by FACS showed that (86.65% ± 2.8%) of P5 hBMSCs was in the G₀/G₁ phase and (14.35% ± 2.8%) was in the S + G₂/M phase. (c) Flow cytometry analysis disclosed that more than 90% of P5 hBMSCs were positive for CD44, CD105, CD166, and CD73; however, they were negative for CD34, CD31, and CD45. (d) Immunofluorescence staining revealed that P5 hBMSCs expressed the antigens of CD73, CD44, CD105, and CD166. (e) hBMSCs differentiated into adipose cells that formed lipid droplets in the cytoplasm, indicated by positive Oil Red staining (upper). The differentiation of hBMSCs to bone was demonstrated by positive von Kossa staining (bottom left). The differentiation to cartilage was reflected by positive Alcian blue staining (bottom right). Scale bar, 50 μm.

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