Nox2 Activity Is Required in Obesity-Mediated Alteration of Bone Remodeling

Figure 2

Effect of Nox2 on high-fat-diet- (HFD-) induced changes in bone mineral density (BMD). Eight-week-old WT and Nox2-KO mice were fed with either a standard lab chow diet (SD) or HFD for 3 months. Mice were scanned with dual-energy X-ray absorptiometry (DXA) before and after administration of experimental diets. (a) Percent changes in BMD before and after experimental diets in different bone regions. (b) Percentage change of the BMD difference between mice fed with an SD and an HFD in different bone regions. Data are expressed as mean ± SEM (, 4 mice/group). In (a), two-way ANOVA followed by post hoc Tukey’s multiple comparison test was used to assess differences among groups with significance defined as . ^#Significantly different from all the group data in the graph. ^§Significantly different from all the group data in the graph. Significantly different from SD-KO in all the graphs. In (b), unpaired -test with Welch’s correction has been used to assess significance between the groups with significance defined as . Significantly different from each other in all the graph data.

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