Potential Phototoxicity of Indocyanine Green in Retinal Pigment Epithelial Cells after Angiography under Ambient Illumination

<div>Phototoxicity of ICG exposed to RPE cells under illumination. Cells were cultured with 0 to 10 <i>μ</i>g/mL ICG in the dark or under illumination for 24 hours. (a) Cell viability and (b) cell death rate are shown. Cell viability of each culture measured by MTS assay was normalized to that of 0 <i>μ</i>g/mL ICG. (c–f) Phase-contrast micrographs. (g, h) Fluorescence micrographs of PI staining and TUNEL. Quantitation of PI-positive cells (k) and TUNEL-positive cells (l) in 10,000 <i>μ</i>m<sup>2</sup>. At least four squares of each experiment were analyzed. 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Oxidative Medicine and Cellular Longevity

fig2

Figure 2

Figure 2: Potential Phototoxicity of Indocyanine Green in Retinal Pigment Epithelial Cells after Angiography under Ambient Illumination 